No ke aha i hoʻohana ʻia ai ka Polyacrylamide no ka electrophoresis protein

Ma ke aupuni o ka ʻepekema hou, kū ka electrophoresis protein ma ke ʻano he ʻenehana pōhaku kihi no ka nānā ʻana a me ke ʻano o nā protein.Aia ma ka puʻuwai o kēia ʻano hanaPolyacrylamide, He hui nui e lawelawe ana ma ke kuamoʻo o nā matrices gel i hoʻohana ʻia i nā ʻōnaehana electrophoresis gel.ʻO nā waiwai kūʻokoʻa o Polyacrylamide he mea pono loa ia no ka poʻe noiʻi a me nā ʻepekema e ʻimi nei e wehe i ka paʻakikī o nā protein a me kā lākou pilina.

ʻO Polyacrylamide, i kapa pinepine ʻia ʻo PAM, he polymer synthetic i hana ʻia mai nā monomers acrylamide.Hāʻawi ʻia kāna versatility kupaianaha i kona hiki ke hana i nā kaulahao lōʻihi, ka hopena i kahi mea like-gel e hiki ke hoʻokipa i nā ʻano nui o nā mole.Hana kēia waiwai i ka polyacrylamide i moho kūpono no ka hana ʻana i nā matrices porous i hoʻohana ʻia i ka electrophoresis protein.

ʻO ka electrophoresis protein kahi hana e hoʻokaʻawale i nā protein e pili ana i kā lākou uku a me ka nui.Ma ke kau ʻana i kahi laʻana protein i kahi māla uila i loko o ka polyacrylamide gel matrix, neʻe nā protein ma o ka gel ma nā ʻano like ʻole, e hopena i nā ʻāpana ʻokoʻa hiki ke nānā ʻia a helu ʻia.Hāʻawi kēia hoʻokaʻawale ʻana i nā ʻike koʻikoʻi i ka maʻemaʻe o ka protein, ka hoʻoholo ʻana i ke kaumaha molecular, a me ke alo o nā isoforms.

ʻO ke kuleana o ka Polyacrylamide i ka Protein Electrophoresis

ʻO ke koho ʻana o ka polyacrylamide no ka electrophoresis protein ke kumu i kona ʻano tunable.Hiki i nā kānaka ʻepekema ke hoʻololi i ka manaʻo o ka matrix gel e hoʻokipa i nā protein o nā ʻano nui like ʻole.Hoʻokumu ʻia nā matrices kiʻekiʻe aʻe i kūpono no ka hoʻonā ʻana i nā protein liʻiliʻi, ʻoiai hoʻohana ʻia nā haʻahaʻa haʻahaʻa no nā protein nui.ʻO kēia hiki ke hōʻoia e hiki i nā mea noiʻi ke hoʻololi i kā lākou mau hoʻokolohua e hoʻokō i ka hoʻokaʻawale maikaʻi a me ka nānā ʻana.

ʻO Polyacrylamide ma ke ʻano heFlocculant

ʻOi aku ka pono o Polyacrylamide ma mua o kāna hana i ka electrophoresis gel.Loaʻa iā ia nā noi ma ke ʻano he flocculant i nā ʻoihana like ʻole, e like me ka mālama wai a me ka hoʻokele wai.Ma ke ʻano he flocculant, kōkua ka polyacrylamide i ka hōʻuluʻulu ʻana i nā mea i hoʻokuʻu ʻia i loko o ka wai, e hoʻomaʻamaʻa i kā lākou lawe ʻana.Hōʻike kēia hiʻohiʻona i nā mana like ʻole o ka pūhui a me ka hopena ākea i ka ʻepekema a me ka ʻoihana.

Nā holomua i ka Polyacrylamide-Based Electrophoresis

Ua ʻike nā makahiki i hala aku nei i ka holomua mau ʻana i nā ʻenehana electrophoresis e pili ana i ka polyacrylamide.Native PAGE, SDS-PAGE, a me ʻelua-dimensional gel electrophoresis he mau hiʻohiʻona wale nō ia o ke ʻano o ka hoʻololi ʻana o polyacrylamide i hiki ai ke hoʻomohala ʻana i nā ʻano hana kūikawā no ka nānā ʻana i nā kūkulu protein, nā hoʻololi post-translational, a me nā pilina.He waiwai nui kēia mau ʻenehana i ka noiʻi proteomic a me nā hana ʻimi lāʻau.

I ke aupuni o ka ʻimi ʻana i ka protein, puka mai ka polyacrylamide ma ke ʻano he hoa paʻa, e hiki ai i nā mea noiʻi ke komo i loko o ka honua paʻakikī o nā protein.ʻAʻole hiki ke hoʻohālikelike ʻia kāna hana ma ke ʻano he kumu o nā matrices gel i nā ʻōnaehana electrophoresis.Mai ka wehe ʻana i nā hana maʻi a hiki i ka hoʻomohala ʻana i nā mea lapaʻau hou, hoʻomau ka electrophoresis-based polyacrylamide i ka holomua ʻepekema.Ke holomua nei ka ʻenehana, e ulu aʻe kēia mea kupanaha synthetic, e hoʻonui hou i ko mākou ʻike i nā protein a me kā lākou mau hana he nui.