No ke aha i hoʻohana ʻia ai ka Polyacrylamide no ka electrophoresis protein

I ke au o ka ʻepekema hou, kū ka electrophoresis protein ma ke ʻano he ʻenehana kihi no ka nānā ʻana a me ke ʻano o nā protein. Aia ma ka puʻuwai o kēia ʻano hanaPolyacrylamide, kahi hui like ʻole e lawelawe ana ma ke ʻano he iwi kuamoʻo o nā matrices gel i hoʻohana ʻia i nā ʻōnaehana electrophoresis gel. ʻO nā waiwai kūikawā o Polyacrylamide e lilo ia i mea hana pono ʻole no nā mea noiʻi a me nā kānaka ʻepekema e ʻimi nei e wehewehe i nā paʻakikī o nā protein a me kā lākou pilina.

ʻO Polyacrylamide, i kapa pinepine ʻia ʻo PAM, he polymer synthetic i hana ʻia mai nā monomers acrylamide. ʻO kona versatility kupaianaha i hāʻawi ʻia i kona hiki ke hana i nā kaulahao lōʻihi, e hopena ana i kahi mea like me ka gel e hiki ke hoʻokipa i nā nui like ʻole o nā molekala. ʻO kēia waiwai e hoʻolilo i ka polyacrylamide i moho kūpono no ka hana ʻana i nā matrices porous i hoʻohana ʻia i ka electrophoresis protein.

ʻO ka electrophoresis protein kahi ʻenehana e hoʻokaʻawale i nā protein ma muli o ko lākou uku a me ka nui. Ma ke kau ʻana i kahi hāpana protein i kahi kahua uila i loko o kahi matrix gel polyacrylamide, neʻe nā protein ma o ka gel ma nā wikiwiki like ʻole, e hopena ana i nā hui like ʻole e hiki ke kālailai ʻia a helu ʻia. Hāʻawi kēia hoʻokaʻawale ʻana i nā ʻike waiwai nui i ka maʻemaʻe o ka protein, ka hoʻoholo ʻana i ke kaumaha molekala, a me ke alo o nā isoforms.

Ke Kūlana o Polyacrylamide ma ka Protein Electrophoresis

ʻO ke koho ʻana o ka polyacrylamide no ka electrophoresis protein ua paʻa i kona ʻano hiki ke hoʻololi ʻia. Hiki i nā kānaka ʻepekema ke hoʻoponopono i ka nui o ka matrix gel e hoʻokipa i nā protein o nā nui like ʻole. ʻO nā nui kiʻekiʻe e hana i nā matrices paʻa i kūpono no ka hoʻonā ʻana i nā protein liʻiliʻi, ʻoiai e hoʻohana ʻia nā nui haʻahaʻa no nā protein nui. Hōʻoia kēia hiki ke hoʻololi i nā mea noiʻi e hiki ke hoʻopilikino i kā lākou mau hoʻokolohua e hoʻokō i ka hoʻokaʻawale a me ka nānā ʻana maikaʻi loa.

ʻO Polyacrylamide ma ke ʻano heMea hoʻoheheʻe wai

ʻOi aku ka pono o ka Polyacrylamide ma mua o kona kuleana i ka electrophoresis gel. Loaʻa iā ia nā noi ma ke ʻano he flocculant i nā ʻoihana like ʻole, e like me ka mālama wai a me ka hoʻokele wai ʻino. Ma ke ʻano he flocculant, kōkua ka polyacrylamide i ka hōʻuluʻulu ʻana i nā ʻāpana i hoʻokuʻu ʻia i loko o nā wai, e hoʻomaʻalahi ana i ko lākou wehe ʻana. Hōʻike kēia ʻano i nā hiki like ʻole o ka hui a me ka hopena ākea i ka ʻepekema a me ka ʻoihana.

Nā Holomua i ka Electrophoresis ma muli o Polyacrylamide

Ua ʻike ʻia nā holomua mau i nā makahiki i hala iho nei i nā ʻano hana electrophoresis e pili ana i ka polyacrylamide. ʻO ka PAGE Native, SDS-PAGE, a me ka electrophoresis gel ʻelua-dimensional he mau laʻana wale nō ia o ke ʻano o ka hiki ke hoʻololi ʻia o ka polyacrylamide i ka hoʻomohala ʻana i nā ʻano hana kūikawā no ka nānā ʻana i nā ʻano protein, nā hoʻololi ma hope o ka unuhi ʻana, a me nā pilina. He waiwai nui kēia mau ʻano hana i ka noiʻi proteomics a me nā hana ʻike lāʻau.

Ma ke kahua o ka loiloi protein, kū mai ka polyacrylamide ma ke ʻano he hoa kūpaʻa, e hiki ai i nā mea noiʻi ke luʻu i loko o ke ao paʻakikī o nā protein. ʻAʻole hiki ke hoʻonui ʻia kona kuleana ma ke ʻano he kumu o nā matrices gel i nā ʻōnaehana electrophoresis. Mai ka wehe ʻana i nā ʻano hana maʻi a hiki i ka hoʻomohala ʻana i nā therapeutics hou, ke hoʻomau nei ka electrophoresis polyacrylamide i ka holomua ʻepekema. I ka holomua ʻana o ka ʻenehana, e ulu paha kēia mea kupanaha synthetic, e hoʻonui hou ana i ko mākou ʻike i nā protein a me kā lākou mau hana he nui.